Fig. 1

Improvement of fibrosis and inflammation in mice by L. crispatus. (A)The morphological changes of intrauterine adhesion were observed in the isolated uterine model; He staining was used to observe inflammatory exudation and Masson staining was used to assess collagen fiber deposition in the uterine tissue of intrauterine adhesion mice (magnification: × 100). At the protein level, the effect of L. crispatus on the expression of (B) TNF-α and (C) IL-1β in uterine tissues of mice with intrauterine adhesions. At the gene level, the effect of L. crispatus on (D) TNF-α and (E) IL-1β transcription in uterine tissues of mice with intrauterine adhesion. (F) Inflammatory protein expression in uterine tissues of mice with intrauterine adhesion. Effects of L. crispatus on inflammation-related (G) TLR4 and (H) p-NF-κB/NF-κB proteins in uterine tissues of mice with intrauterine adhesion. C group, Control group; M group, Mechanical injury was used to construct a model of intrauterine adhesion; L group was treated with L. crispatus for intrauterine adhesion mice. *P < 0.05; **P < 0.01; ***P < 0.001. Western blotting images were cropped to improve the conciseness of the data, and the original images with visible membrane edges can be found in Supplementary Material