Fig. 6

Stress response pathways associated with cell wall biogenesis and remodeling were constitutively upregulated in the Δcdc50 mutant. A Mid-log phase yeast was collected, washed, and frozen to isolate total RNA, followed by qRT-PCR. The relative expression of genes in different strains were obtained by comparison with that in the wild-type (WT). In the Δcdc50 mutant, the genes that encode the constituents of the cell integrity pathway, including Slt2, Rlm1, Swi4, Swi6, Fks1, Fks2 and Chs3, all showed increased expression to varying degrees. B Mid-log phase WT and Δcdc50 yeast were collected for complex transcriptome sequencing, and the expression level of every gene was quantified using the TPM method. Differentially expressed genes were enriched via GO functional enrichment analysis to identify significantly different GO pathways between the WT and Δcdc50. The top 12 most significant pathways were mainly implicated in cell wall organization and biogenesis. C The TPM of genes enriched in the GO pathways in B were gathered and taken the logarithm to generate a heatmap for visualized comparison. TPM, transcripts per million reads; GO, Gene Ontology. *, P < 0.05; **, P < 0.01; ***, P < 0.001