Fig. 3

Increased cell wall mannan, chitin and β-1,3-glucan contents, along with exposed chitin and β-1,3-glucan were found in Δcdc50. A Yeast cells in mid-log phase were collected and incubated in FITC-ConA, CFW and Aniline Blue to assess the total cell wall mannan, chitin, and β-1,3-glucan contents, respectively, then analyzed using flow cytometry and spectrofluorometry. In the Δcdc50 mutant, the cell wall mannan content increased by 14.5%, total chitin contents increased significantly by 39%, and total β-1,3-glucan contents increased by 44.3%. B Exposure of chitin and β-1,3-glucan were tested using FITC-WGA and anti-β-1,3-glucan antibodies. The exposure of inner chitin has increased by 14.9%, while the β-1,3-glucan exposure has increased by 25.5% in the Δcdc50 mutant. Data represent the mean ± SD of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001