Fig. 2
From: P4-ATPase subunit Cdc50 plays a role in yeast budding and cell wall integrity in Candida glabrata
The Δcdc50 mutant showed hypersensitivity to antifungal drugs and cell wall stressors. Mid-log phase yeast was adjusted to 0.1 OD at 600 nm and diluted tenfold serially to be spotted on YPD plates containing different drugs. A The Δcdc50 mutant exhibited weakened resistance to the clinically used antifungal drugs fluconazole, itraconazole and caspofungin. FLC, fluconazole; ICZ, itraconazole; CSF, caspofungin. B The Δcdc50 mutant was more sensitive to cell wall stressors CR, CFW and SDS, suggesting cell wall remodeling. CR, Congo Red; CFW, Calcofluor White; SDS, sodium dodecyl sulfate