Fig. 1

The Δcdc50 mutant showed defects in cell growth, yeast budding, and actin polarization. A Wild-type (WT), Δcdc50, and Δcdc50 + CDC50 strains were adjusted to the same cell density and incubated in fresh YPD medium at 30 ℃ for 24 h. Their OD at 600 nm was recorded to construct the cell growth curve. In the exponential phase, the Δcdc50 mutant exhibited significantly slower growth compared with the other two strains. B The cell cycle distribution of different strains as determined using microscopy. Mid-log phase yeast was collected and stained to be classified as having no, small, or large buds. More than 300 cells were counted in each group. The Δcdc50 mutant showed increased numbers of small buds and decreased numbers of large buds compared with those of the wild-type. Data represent the mean ± SD of three independent experiments. C Typical views of wild-type and Δcdc50 actin fluorescent imaging during yeast budding. After staining with FITC-phalloidin, the WT and Δcdc50 yeast cells were observed using fluorescence microscopy. Most WT budding yeasts exhibited normal actin patch polarization (enriched in buds and bud necks), while a large amount of Δcdc50 budding yeasts showed actin mislocalization: The actin patches were depolarized and scattered. Bar, 10 μm. *, P < 0.05. Data represent the mean ± SD of three independent experiments