Table 2 Summary of microorganisms of all genera detected by Modified Chip, Direct Plating and Miseq high-throughput sequencing (“-“means not detected)
From: Use of modified ichip for the cultivation of thermo-tolerant microorganisms from the hot spring
Genus | Proportion (Miseq high-throughput sequencing) (%) | Direct plating methods | Modified ichip first generation | Modified ichip second generation |
|---|---|---|---|---|
Actinotalea | – | – | 1 | – |
uncultured Actinotalea | – | – | 1 | – |
uncultured Lysobacter | – | – | 7 | 1 |
Alkalihalobacillus | – | – | 4 | – |
Pannonibacter | 21.0689 | – | 5 | – |
Pseudomonas | – | – | 13 | 26 |
Hyphomicrobium | – | – | 1 | – |
Bosea | – | – | 1 | – |
Microbacterium | 0.0504 | – | 1 | – |
Kocuria | – | – | 1 | – |
uncultured Aquiflexum | – | – | – | 2 |
Ochrobactrum | – | – | – | 2 |
Brucella | – | – | – | 2 |
Brevibacillus | – | – | – | 1 |
Agromyces | – | 2 | 6 | 4 |
Bacillus | 45.2028 | 3 | 3 | 5 |
Brevibacterium | 6.6772 | 13 | – | – |
Geobacillus | – | 3 | – | – |
Paenibacillus | 1.1345 | 1 | – | 3 |
Sphingomonas | – | 4 | 1 | 2 |
Alkalicoccus | 0.1152 | – | – | – |
Diaphorobacter | 0.0216 | – | – | – |
Pajaroellobacter | 0.0108 | – | – | – |
Phenylobacterium | 0.4322 | – | – | – |
Parabacteroides | 0.2197 | – | – | – |
Paracoccus | 0.0612 | – | – | – |
Muribaculum | 0.2305 | – | – | – |
Akkermansia | 1.6423 | – | – | – |
Alicyclobacillus | 0.2917 | – | – | – |
Prevotella | 0.0972 | – | – | – |
Hydrogenobacter | 8.107 | – | – | – |
Egicoccus | 0.6663 | – | – | – |
Alloprevotella | 0.4358 | – | – | – |
Eubacterium | 0.2161 | – | – | – |
Mogibacterium | 0.2089 | – | – | – |
Roseburia | 0.2017 | – | – | – |
Alicycliphilus | 0.7383 | – | – | – |
Alistipes | 0.479 | – | – | – |
Rubrobacter | 0.1369 | – | – | – |
Nitriliruptor | 7.8441 | – | – | – |
Hydrogenophilus | 0.1044 | – | – | – |
Rhodococcus | 0.0432 | – | – | – |
Bacteroides | 2.0529 | – | – | – |
Blautia | 0.5186 | – | – | – |
Other bacterium | – | – | 2 | 11 |
Other uncultured bacterium | 0.479 | – | – | 1 |