Fig. 3

Inhibition of C. perfringens culture supernatants (a PDR isolate from chicken breast muscle) hemolysis activity upon incubation with MYR, ZnO, ZnO/PVA, and ZnO/PVA/MYR. A) Representative image of bacterial supernatants pre-incubated with varying concentrations (MIC/4, MIC/2, MIC, 2X MIC, 4X MIC) of MYR, ZnO, ZnO/PVA, and ZnO/PVA/MYR compared to untreated positive control and un-inoculated negative control, then the hemolysis activity was determined. B) Inhibition of hemolysis activity was performed with varying concentrations and calculated as hemolysis percentage. The half maximal inhibitory concentration (IC50, μg/mL) was represented for each treatment (0.6, 1.3, 0.4, and 0.04). The experiments were done in triplicate and each column represented the mean ± SEM. Different small letters on the same coloured column indicate significant difference (P < 0.05)