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Table 4 Quantification of bacterial numbers present in multi-species probiotic blends

From: Insights into the enumeration of mixtures of probiotic bacteria by flow cytometry

 

FC

PC

p-values (FC vs PC)

Formulated

AFU/g

Recovered AFU/g

RSD

(%)

% Recovery

Formulated

CFU/g

Recovered CFU/g

RSD

(%)

% Recovery

Recovered counts/g

% Recovery

Blend 1

3.85 × 1010

3.66 × 1010

9.62

95.22

3.00 × 1010

2.78 × 1010

10.86

92.75

0.0054

< 0.0001

Blend 2

3.62 × 1010

4.04 × 1010

7.34

111.56

3.02 × 1010

1.76 × 1010

14.60

58.18

0.0001

0.0003

Blend 3

4.33 × 1010

4.30 × 1010

6.86

99.43

3.39 × 1010

3.10 × 1010

15.62

91.40

< 0.0001

0.0088

Blend 4

4.30 × 1010

4.35 × 1010

3.44

101.08

3.33 × 1010

2.76 × 1010

11.47

83.13

< 0.0001

0.0010

Mean (SD)

  

6.82 (2.55)

101.8 (6.945)

  

13.14 (2.33)

81.37 (16.03)

  
  1. Data represents the mean of at least 5 experimental repeats. Values of p were determined using the Student’s paired t-test
  2. Abbreviations: AFU Active fluorescence units, CFU Colony forming units, RSD Relative standard deviation, FC Flow cytometry, PC Plate count, SD Standard deviation
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BMC Microbiology

ISSN: 1471-2180

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