Fig. 6

Motility phenotype of wt, ΔsigB, and Δbc1009 mutants. A- the motility of wt, ΔsigB, and Δbc1009 was compared on Brain Heart Infusion (BHI) agar with 0.25% agar and indicated by the colony diameter (mm) formed on the agar after 24 h incubation. Black bar: wt; Grey bar: ΔsigB mutant; white bar: Δbc1009 mutant; light grey bar: ΔflgG mutant (negative control without flagella). The motility of all cells was tested under three different conditions, 1) at 30 °C for 24 h; 2) upon heat shock from 30 °C to 42 °C for 30 min, and back to 30 °C for 24 h; and 3) at 42 °C for 24 h. The dotted line shows the maximum plate size. B- Colony of wt, ΔsigB, Δbc1009, and ΔflgG cells on 0.25% BHI agar at 30 °C after 24 h. Error bars indicates the standard deviation of colony diameters of four biological replicates