Fig. 3
SigB and SigB (and Bc1009)-dependent induced and downregulated proteins in wt cells after heat shock (30 °C to 42 °C) versus before heat shock and their cluster of orthologous group (COG). A- log2 fold change in protein expression. Positive and negative fold change values indicate induced and downregulated proteins, respectively. Numbers indicate the top 30 induced (Table 2) or top 20 downregulated (Table 3) proteins, respectively. The p-value threshold for each protein was < 0.05, N = 4. Grey symbols: induced/downregulated proteins that are SigB (and Bc1009-dependent; i.e., proteins that show up(or down) regulation of > 0.6 log2 fold change in wt/ΔsigB and wt/Δbc1009 cells upon heat shock vs. non-heat-stressed condition. White symbols: induced/downregulated proteins that are SigB-dependent; i.e., proteins that show up(or down) regulation of > 0.6 log2 fold change in wt/ΔsigB cells upon heat shock vs. non-heat-stressed condition. B- cluster of orthologous group (COG) function for both SigB-dependent induced (Right) and downregulated proteins (Left) in wt cells upon heat shock. The number on the x-axis indicates the total number of induced/downregulated proteins, and the negative sign indicates downregulation. Grey bar: induced/downregulated proteins after heat shock that are SigB (and Bc1009)-dependent, i.e., proteins that show up(or down) regulation of > 0.6 log2 fold change in wt/ΔsigB and wt/Δbc1009 cells; White bar: induced/downregulated proteins that are SigB-dependent, i.e., proteins that show up(or down) regulation of > 0.6 log2 fold change only in wt/ΔsigB cells respectively, compared to the expression in the non-heat-stressed condition at 30 °C. The underlying transcriptome data supporting this figure are presented in Supplementary Table S3B