Fig. 2
Proteomics analyses of B. cereus ATCC14579 wt, ΔsigB, and Δbc1009 mutants upon heat shock. Left: Venn diagram showing 429 significantly induced (upregulated) proteins in B. cereus heat-stressed wt cells (black circle) compared to the non-heat-stressed wt cells at 30 °C (see Supplementary Table S2A). White circle: 175 heat-induced proteins in wt that are SigB-dependent, i.e., proteins that show upregulation of > 0.6 log2 fold change in wt/ΔsigB cells upon heat shock compared to the non-heat-stressed condition at 30 °C. Grey circle: For 98 proteins, the heat-mediated increase in level in the wt is dependent on SigB and Bc1009, i.e., proteins that show upregulation of > 0.6 log2 fold change in wt/ΔsigB and wt/Δbc1009 cells upon heat shock compared to the non-heat-stressed condition at 30 °C (see Table 2 for the complete list of proteins). Right: Venn diagram showing 435 significantly downregulated proteins in B. cereus heat-stressed wt cells (black circle) compared to the non-heat-stressed wt cells at 30 °C (Supplementary Table S2A). White circle: 109 downregulated proteins in heat-stressed wt cells vs. non-heat-stressed wt cells are SigB-dependent, i.e., proteins that show downregulation of > 0.6 log2 fold change in wt/ΔsigB cells upon heat shock compared to the non-heat-stressed condition at 30 °C. Grey circle: For 40 proteins, reduction in level in wt cells upon heat shock was dependent on SigB and Bc1009, i.e., proteins that show downregulation of > 0.6 log2 fold change in wt/ΔsigB and wt/Δbc1009 cells upon heat shock vs. non-heat-stressed condition (see Table 3). The underlying transcriptome data supporting this figure are presented in Supplementary Table S2B