Fig. 2

Bacterial co-occurrence and differential abundance in cloacal microbiota of larks from different geographic locations. (A) Shared and unique bacterial ASVs and genera in the cloacal microbiota of larks compared among geographic locations. For visual clarity genus is abbreviated as “G” and ASV as “A”, and overlapping fields without text represent < 1% shared ASVs and genera. Co-occurrences are based on rarefied data of 4000 reads per sample, and differentially abundant ASV (n = 52) were determined using ANCOM-BC on samples with more than 1000 reads and ASVs with at least 10% non-zero counts. (B) Distinct clustering of differentially abundant ASVs among locations