Table 2 Comparison of results of eight types of cryopreservation conditions for two species of Astrephomene based on most probable number (MPN) methods
From: Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene
Conditions for cryopreservation (species) | Total viabilitya (range) % | Experiment I | Experiment II |
|---|---|---|---|
MPN cell numbers in three cryotubes (/mL) (control); [Number of viable cultures by 1st (2nd) inoculation with 3 (3) 10 mL cultures] | MPN cell numbers in three cryotubes (/mL) (control); [Number of viable cultures by 1st (2nd) inoculation with 3 (3) 10 mL cultures] | ||
Immature colonies in 3% DMF (AGb) | 0.36 ± 0.38 (0.022-1.0) | 92, 92, 230 (22000); [3(2)] | 9.2, 23, 92 (42000); [3(0)] |
Immature colonies in 6% DMF (AG) | 11 ± 13 (0.36-33) | 2300, 4200, 7300 (22000); [3(3)] | 150, 230, 230 (42000); [3(3)] |
Immature colonies in 3% HA (AG) | 0.24 ± 0.20 (0.014-0.46) | 3, 7.3, 74 (22000); [3(1)] | 140, 420, 420 (92000); [3(3)] |
Immature colonies in 6% HA (AG) | 0 ± 0 (0-0) | 0, 0, 0 (22000); [0(0)] | 0, 0, 0 (92000); [0(0)] |
Mature colonies in 3% DMF (AG) | 0 ± 0 (0-0) | 0, 0, 0 (22000); [0(0)] | 0, 0, 0 (9200); [0(0)] |
Mature colonies in 6% DMF (AG) | 0 ± 0 (0-0) | 0, 0, 0 (22000); [2(0)] | 0, 0, 0 (9200); [0(0)] |
Mature colonies in 3% HA (AG) | 0 ± 0 (0-0) | 0, 0, 0 (22000); [2(1)] | 0, 0, 0 (14000); [3(0)] |
Mature colonies in 6% HA (AG) | 0 ± 0 (0-0) | 0, 0, 0 (22000); [0(0)] | 0, 0, 0 (14000); [0(0)] |
Immature colonies in 3% DMF (APc) | 0.053 ± 0.098 (0-0.25) | 23, 42, 230 (92000); [3(2]] | 0, 0, 0 (9200); [0(0)] |
Immature colonies in 6% DMF (AP) | 0.032 ± 0.037 (0-0.1) | 9.2, 9.2, 92 (92000); [3(1)] | 0, 3, 3.6 (9200); [3(1)] |
Immature colonies in 3% HA (AP) | 2.2 ± 1.9 (0.3-4.6) | 920, 1500, 4200 (92000); [3(3)] | 28, 92, 420 (9200); [3(3)] |
Immature colonies in 6% HA (AP) | 0.071 ± 0.077 (0-0.14) | 0, 0, 0 (92000); [0(0)] | 13, 13, 13 (9200); [3(3)] |
Mature colonies in 3% DMF (AP) | 0 ± 0 (0-0) | 0, 0, 0 (7400); [1(0)] | 0, 0, 0 (9200); [0(0)] |
Mature colonies in 6% DMF (AP) | 0.093 ± 0.12 (0-0.31) | 9.2, 9.2, 23 (7400); [3(1)] | 0, 0, 0 (9200); [3(0)] |
Mature colonies in 3% HA (AP) | 5.5 ± 5.9 (0.12-12) | 740, 740, 920 (7400); [3(3)] | 11,15, 21 (9200); [3(3)] |
Mature colonies in 6% HA (AP) | 0 ± 0 (0-0) | 0, 0, 0 (7400); [0(0)] | 0, 0, 0 (9200); [0(0)] |