Table 2 Bacterial strains and plasmids used in this study
Strains or plasmids | Description | Source or reference |
|---|---|---|
Strains | ||
 E. coli DH5α | F−endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoRnupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK−mK+) phoA λ− | Takara |
 Escherichia coli WM3064 | thrB1004 pro thirpsLhsdS lacZDM15 RP4–1360 (araBAD)567dapA1341::[ermpir(wt)] | W. Metcalf |
 E. coli EC100D+ | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galUgalK λ−rpsL (StrR) nupGpir+(DHFR) | Epicentre Technologies |
 RN12WT | Floc-forming strain | (3) |
 RN12T4 mutant | mariner transposon insertion in rpoN1 at nt 1274 | (3) |
 RN12M35 mutant | mariner transposon insertion in epsB2 at nt 1295 | This study |
 RN12M51 mutant | mariner transposon insertion in prsT at nt 2097 | This study |
plasmids | ||
 pminiHmarRB1 | The mariner transposon delivery plasmid R6K | (15) |
 pBBR1MCS-2 | Broad-host-range shuttle vector, Kmr | (20) |
 pBBR1MCS-5 | Broad-host-range cloning vector, Gmr | (20) |
 pBBR1MCS2-rpoN1 | rpoN1 gene cloned in pBBR1MCS-2, Kmr | (3) |
 pBBR1MCS2-pepA | pepA gene cloned in pBBR1MCS-2, Km | This study |
 pBBR1MCS-5-prsT | prsT gene cloned in pBBR1MCS-5, Gmr | This study |
 pBBR1MCS-5-epsB2 | epsB2 gene cloned in pBBR1MCS-5, Gmr | This study |