Fig. 8

The overexpression of PEP-CTERM gene pepA did not rescue the swarming motility and biofilm formation of the rpoN1 disruptant of A. tertiaricarbonis.A Colony mophology of each strain after three day incubation. Scale bar: 1 cm; B Swarming motility expressed as colony diameters at 24 h, 48 h and 72 h; C Biofilm levels measured after 24 h, 36 h and 48 h incubation at 28 °C in the R2A media. Each sample was plated in triplicate. Biofilms were stained with 0.1% crystal violet (CV). CV was dissolved with 30% acetic acid and quantified by measuring the optical density at 595 nm; Error bars represent the standard deviation