Fig. 5

Overexpression of the PEP-CTERM gene pepA circumvented the requirement of RpoN1 for floc formation in the RN12T4 mutant. Plasmid borne wild type pepA gene recovered the floc-forming phenotype of the RN12T4 mutant similar to that of the wild type strain RN12 with the empty vector. A Wild type RN12 strain carrying the pBBR1MCS-2 empty vector. B RN12T4 mutant with pBBR1MCS-2 empty vector. C RN12T4 mutant with pBBR1MCS-2-rpoN1 construct. D RN12T4 mutant with pBBR1MCS-2-pepA construct. E Agitated bacterial cultures from panels A to D, from left to right. F Settled bacterial cultures from panels (A) to (D), from left to right. A to D were visualized by light microscope after staining with methylene blue (× 1000 magnification); Bars: 10 μm