Optimization of glutathione production in Saccharomyces cerevisiae HBSD-W08 using Plackett–Burman and central composite rotatable designs

Table 7 Central Composite Test Design and results

Number	Peptone/(g L^− 1)	KH₂PO₄/(g L^− 1)	Glutamic acid/(g L^− 1)	GSH yield/(g L^− 1)
1	2.00	0.000	0.075	3.6186
2	2.00	0.545	0.075	3.4088
3	2.00	0.250	0.000	2.1884
4	2.50	0.250	0.151	1.1587
5	2.50	0.125	0.100	3.7330
6	2.50	0.375	0.050	3.1037
7	2.50	0.375	0.100	3.5614
8	2.50	0.125	0.050	3.4279
9	1.50	0.375	0.100	2.9703
10	1.50	0.375	0.050	3.1991
11	0.14	0.250	0.075	1.7880
12	3.86	0.250	0.075	2.9893
13	1.50	0.125	0.050	3.3326
14	1.50	0.125	0.100	3.4470
15	2.00	0.250	0.075	3.6681

ISSN: 1471-2180