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Fig. 5 | BMC Microbiology

Fig. 5

From: Investigation on the applicability of a long-range reverse-transcription quantitative polymerase chain reaction assay for the rapid detection of active viruses

Fig. 5

Effect of 0.12% sodium hypochlorite on IAV (PR8 strain) titers and copy numbers. IAV suspensions were treated with 0.12% sodium hypochlorite for increasing periods between 3 and 10 min. The titers of treated IAVs were determined by TCID50 assay using MDCK cells. The copy numbers of treated IAV were measured by LR-RT-qPCR targeting the PA segment and by conventional RT-qPCR targeting the M segment, respectively. The titers are plotted on the graph and shown as log (TCID50/0.1 mL) (◆). Assays were carried out independently three times with quadruplicate wells per dilution and values represent the mean ± standard error. After over 3 min treatment, there was no CPE in all conditions. They were defined as 0 (means ‘Not detected’) for convenience. The ratios of copy numbers obtained using LR-RT-qPCR and conventional RT-qPCR are also plotted on the graph (● and ▲), respectively. Assays were performed with three different lots of the PR8 stocks and plots represent the mean ± standard error and individual values. A broken line indicated the LoD of LR-RT-qPCR. The data indicated in Additional Table 5 (See ‘Additional Tables’)

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BMC Microbiology

ISSN: 1471-2180

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