Skip to main content
Fig. 3 | BMC Microbiology

Fig. 3

From: Investigation on the applicability of a long-range reverse-transcription quantitative polymerase chain reaction assay for the rapid detection of active viruses

Fig. 3

Effect of 70% ethanol on IAV (Panama strain) infectivity and copy numbers. IAV suspensions were treated with 70% ethanol for increasing periods between 10 and 30 min. The titers of treated IAV were measured by TCID50 assay using MDCK cells. The titers are plotted on the graph and shown as log (TCID50/0.1 mL) (◆). Assays were performed independently three times with quadruplicate wells per dilution and values represent the mean ± standard error. After over 10 min treatment, there was no CPE in all conditions. These titers were defined as 0 (means ‘Not detected’) for the convenience of plotting. The copy numbers were measured using LR-RT-qPCR targeting the PA segment. The ratio of copy numbers obtained using LR-RT-qPCR is also plotted on the graph (●). Assays were carried out independently three times with triplicate wells per sample and plots represent the mean ± standard error and individual values. A broken line indicated the LoD of LR-RT-qPCR. The data indicated in Additional Table 3 (See ‘Additional Tables’)

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us