Table 2 Estimated plasmid copy number (PCN) by absolute quantification

Before Cm^d

7.96 ± 0.02

16.23 ± 0.03

1.78 × 10⁸ (2.5%)

6.70 × 10⁵ (1.8%)

245.1 (4.2%)

After Cm

7.34 ± 0.04

16.00 ± 0.09

2.70 × 10⁸ (5.4%)

8.15 × 10⁵ (5.6%)

325.6 (1.7%)

repB

alr

repB

alr

pTE15

Before Cm^d

9.63 ± 0.04

12.33 ± 0.07

5.80 × 10⁷ (4.1%)

9.50 × 10⁶ (5.6%)

6.0 (1.7%)

After Cm

12.27 ± 0.06

13.49 ± 0.03

9.90 × 10⁶ (4.8%)

4.36 × 10⁶ (2.2%)

2.3 (4.3%)