Fig. 2

The role of NagZ in ampC expression and resistance in EC. RT-qPCR (A) and cropped Western Blot (B) verified that nagZ-knockout EC clinical isolate (ΔnagZ) was successfully constructed. C-D RT-qPCR and Western Blot (cropped blots) were employed for the determination of the role of NagZ in the expression of AmpC induced by subinhibitory concentration CFZ (256 μg/ml) and IMP (0.125 μg/ml) in EC clinical isolate (WT), ΔnagZ, ΔnagZ treated with CFZ (ΔnagZ + CFZ) and ΔnagZ treated with IMP (ΔnagZ + IMP). E Western blot Quantitative analysis (D), The internal control employed was DnaK. F Nitrocefin hydrolysis assay in WT, ΔnagZ, ΔnagZ + CFZ, and ΔnagZ + IMP was employed to examine the role of NagZ in AmpC β-lactamase activity. ** P < 0.01 indicates high statistical significance