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Fig. 1 | BMC Microbiology

Fig. 1

From: Cefazolin and imipenem enhance AmpC expression and resistance in NagZ-dependent manner in Enterobacter cloacae complex

Fig. 1

Effects of SICs of CFZ and IMP on AmpC expression and activity in EC clinical isolate. A Analysis of the ampC mRNA expression levels was made using RT-qPCR in EC clinical isolate treated with SICs of CFZ (64 μg/ml and 256 μg/ml) and IMP (0.0625 μg/ml and 0.125 μg/ml). B Western blot (cropped blots) analysis of ampC protein expression in EC clinical isolate treated with SICs of CFZ and IMP. C Quantitative analysis of the western blot results (B) with image J software, DnaK was employed as an internal control. D Determination of the effects of SICs of CFZ and IMP on AmpC activity by nitrocefin hydrolysis assay. ** P < 0.01 suggests the high statistical significance

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