Fig. 3

CDAHFD fed Göttingen minipigs experienced hyperglucagonemia and altered gene expression in the liver. Göttingen Minipigs were fed either a control (n = 5) or choline-deficient amino acid defined high fat diet (CDAHFD) (n = 7) for 8 weeks starting at age 8 weeks. A Body weight (Kg), B Fasting fructosamine (µmol/L) and C Fasting glucagon (pM) (previously published in Pedersen and colleagues (2020) [24]); D Liver-a-cell axis: Glucagon-alanine index (pM/µM); E Relative gene expression (log2 relative quantitives of mRNA) of hepatic glucagon receptor (GCGR) and glucose-6-phosphatase subunit (G6PC) determined by quantitative real-time PCR, fdr adjusted. Data are shown as individual values with mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 mark the level of statistical significance