Fig. 4

Upregulated and downregulated signaling pathways and CAZymes in the MSTN-edited and wild-type groups. A Bray–Curtis-based PCoA of the functional pathways of the GEF and WTF. The P-value was based on ANOSIM. The boxplot for the discrete distribution of samples along the PC1 and PC2 axes. B Significantly changed pathways of the GEF and WTF at level 1 (metabolism pathways) and (C) level 3. The vertical axis represents different pathways, and the horizontal axis represents the proportions of corresponding pathways. (0.01 < P ≤ 0.05 *, 0.001 < P ≤ 0.01 **, P ≤ 0.001 ***). D Bray–Curtis-based PCoA of CAZymes of the GEF and WTF. The P-value is based on the ANOSIM. The boxplot shows the discrete distribution of samples along the PC1 and PC2 axes. E The significantly different CAZymes of gut microbiome between the GEF/GEM and WTF/WTM. The vertical axis represents different CAZymes, and the horizontal axis represents the proportions of corresponding enzymes. (0.01 < P ≤ 0.05 *, 0.001 < P ≤ 0.01 **)