Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

BMC Microbiology

Table 3 Resource requirements and outputs of three DNA extraction methods

Method	Cost per extraction^a	Extraction time	Number of OTUs detected
Method	Cost per extraction^a	Extraction time	0 h	6 h	18 h
PowerFood	$ 5.02	34 min	14 ± 1	13 ± 1	18 ± 2^b
PowerSoil	$ 8.27	36.5 min	14 ± 1	14 ± 1	17 ± 2
Hybrid	$ 10.28	2 h 10 min	13 ± 1	13 ± 1	19 ± 3^c

^acost reflects pricing available to Canadian government laboratories at the time the study was conducted
^bsignificantly higher (P < 0.05) than at 0 h and 6 h, as determined by one-way analysis of variance (ANOVA) with the Tukey post-hoc test, n = 4
^csignificantly higher (P < 0.05) than at 0 h and 6 h, as determined by one-way analysis of variance (ANOVA) with the Tukey post-hoc test., n = 4

ISSN: 1471-2180