Fig. 4
From: Carboxypeptidase G and pterin deaminase metabolic pathways degrade folic acid in Variovorax sp. F1
Enzymatic properties of rCPG derived from Variovorax sp. F1. A SDS-PAGE gel. Lanes: M, molecular weight marker; rCPG, Recombinant CPG (1.8 μg) from Variovorax sp. F1 strain. B–D HPLC analyses of reactions containing 5 μg mL− 1 rCPG and various substrates at 30 °C for 15 min. Substrates (5 mM) each are B FA; C DFA; D ABG. Traces: 1, substrates; 2, products; 3, reaction. E Specific activities of rCPG against FA, DFA, and ABG. Error bars indicate standard errors of means (n = 3). *P < 0.05. ABG, N-(4-aminobenzoyl)-L-glutamic acid; DFA, deaminofolic acid; DPA, deaminopteroic acid; FA, folic acid; PA, pteroic acid; rCPG, recombinant carboxypeptidase G