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Fig. 5 | BMC Microbiology

Fig. 5

From: Individual competence predominates over host nutritional status in Arabidopsis root exudate-mediated bacterial enrichment in a combination of four Burkholderiaceae species

Fig. 5

Experimental design. Procedure to obtain Arabidopsis thaliana root exudate pools (A), used in the sequential transference experiment (B). Plant root exudates were collected from plants growing under standard or nitrogen limitation conditions after 14 or 21 days; 14d.PRE and 21d.PRE, and 14d.N-PRE and 21d.N-PRE, respectively. Eight replicates of the individual or combined inocula of Paraburkholderia phytofirmans PsJN, Cupriavidus pinatubonensis JMP134, C. metallidurans CH34, and C. taiwanensis LMG19424 were grown for 120, or 72 h. Growth curves, viable cell counting, and bacterial abundances were then determined. Three of the eight replicates were selected to inoculate the next generation. This process was repeated until the seventh generation. For more details on the selection procedure, see Additional File 2

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BMC Microbiology

ISSN: 1471-2180

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