Fig. 3
From: Isophthalate:coenzyme A ligase initiates anaerobic degradation of xenobiotic isophthalate
A Purification of recombinant proteins and determination of subunit architecture of IPCL. A SDS-PAGE (12.5% acrylamide) of purified IPCL (375) and hypothetical protein (0374); M, marker. B Analysis of its native molecular weight by native PAGE. C). The plot showing the Tm of IPCL identified by plotting the first derivative of the fluorescence emission as a function of temperature (−dF/dT). Tm is represented as the lowest part of the curve