Table 1 NanoString CodeSet for apoptosis genes in Euprymna scolopes

Acin1

Mediates apoptotic chromatin condensation following caspase 3 activation without fragmentation

Aifm1

Contributes to caspase-independent apoptosis, also activates executioner caspases 7

Aifm3

Implicated in intrinsic, caspase-dependent, apoptosis via reduced mitochondrial membrane potential

Apaf1

Forms the apoptosome complex with cytochrome C

Api5^a

Anti-apoptotic factor that inhibits E2f1-induced apoptosis

Arts

Regulates cytoskeletal organization and is required for Tgf-β induced apoptosis

Aspp1

Enhances the transactivation and DNA-binding activity of p53 at the promoters of pro-apoptotic genes

Atm

Senses DNA damage and activates checkpoint signaling in response to apoptotic or genotoxic stressors

Aven

Inhibits Apaf1-dependent intrinsic apoptosis

Bcl2L1

Blocks caspase activation and the release of cytochrome C from the mitochondria. BclXL isoform

Bag1

Strengthens anti-apoptotic Bcl2 repression, inhibits pro-death PP1R15 phosphatase

Bag4

Prevents constitutive signaling via Tnf superfamily receptor member 1A

Bak

Forms pores in the mitochondrial membrane via oligomerization in response to intracellular stress

Bax

Upon activation results in cytochrome C release and intrinsic apoptosis

Birc6

Targets caspases 3, 7, and 9 as well as mitochondrial-derived Diablo/Smac for proteasome degradation

Bnip3

Overcomes Bcl2-type suppression of pro-death effectors Bax/Bak. Induced by hypoxia-dependent signaling

Casp2

Activated in the DISC via caspase 8, the intrinsic p53-induced PIDDosome, and is cleaved by caspase 3

Casp3

Pro-apoptotic executioner and targets structural proteins and activates effectors to assist in cell breakdown

Casp7^a

Pro-apoptotic executioner and targets structural proteins and activates effectors to assist in cell breakdown

Casp8

Pro-apoptotic initiator and activated during extrinsic, receptor-mediated, apoptosis via DISC assembly

Casp9

Pro-apoptotic initiator and activated via the apoptosome in response to fatal levels of intracellular stress

Casp10

Pro-apoptotic initiator and activated during extrinsic, receptor-mediated, apoptosis via DISC assembly

CatL^a

Lysosomal cysteine protease and degrades pro-death CatD, protects against neuronal apoptosis

Daxx^a

Proposed to mediate apoptosis in response to Fas death receptor stimulation

Diablo

Promotes caspase-dependent apoptosis and suppresses inhibitors of apoptosis after mitochondrial release

Diap2

Inhibitor that inactivates effector caspase Drice (caspase 3 homolog) via ubiquitination

Fadd

Adaptor protein that recruits and activates caspases 8 and 10 in response to Tnfr stimulation

Hsp7c

Up-expression correlates with reduced apoptosis. Binds LPS. High levels inhibit p53 activation

Hsp90

Inducible molecular chaperone that stabilizes Akt kinase, which targets caspase 9 to inhibit apoptosis

Jnk

Phosphorylates transcription factors in response to radiation, including Jun, and promotes apoptosis

Jun

Demonstrated role in promoting apoptosis, antagonized by Bcl2 inhibitor

Lbp1

Binds LPS by the lipid A region. Plays a role in establishing the light organ symbiosis

Lbp2

Binds gram-negative LPS by the lipid A region

Lbp3

Binds gram-negative LPS by the lipid A region

Litaf

p53 inducible, promotes Tnf-α transcription in response to LPS. Inhibits anti-apoptotic Bcl6 and BclXL

Mcl1

Promotes cell survival and inhibits intrinsic apoptosis by blocking Bak/Bax

Pin1

Promotes cellular survival and inhibits pro-apoptotic factors including Bak, Bax, Fadd, and Daxx

RipK1

Activates pro-death Jnk cascade in response to Tnf-α. Also promotes cell survival via NF-κB activation

Traf2

Promotes p53-dependent apoptosis via Jnk activation. Regulates NF-κB

Traf6

Activates NF-κB and contributes to apoptosis through Ask1-dependent Jnk activation

Xiap^a

Inhibitor of apoptosis that targets proteins to proteasome for degradation via ubiquitination