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Table 2 Results of next generation sequencinga

From: Species-specific identification of Pseudomonas based on 16S–23S rRNA gene internal transcribed spacer (ITS) and its combined application with next-generation sequencing

Sample number

Pseudomonas ITS / Total reads

Species1 (G/F)b

Species2 (G/F)

Species3 (G/F)

1

9212 / 51,322

P. putida (F)

37%

P. koreensis (F)

17%

P. fluorescens (F)

6%

2

7321 / 46,231

P. putida (F)

51%

P. monteilii (G)

9%

P. fluorescens (F)

5%

3

8169 / 57,330

P. putida (F)

32%

P. fluorescens (F)

12%

P. koreensis (F)

9%

4

6949 / 37,655

P. putida (F)

21%

P. aeruginosa (F)

7%

P. monteilii (G)

5%

5

9333 / 50,137

P. putida (F)

52%

P. fluorescens (F)

11%

P. monteilii (G)

3%

6

6392 / 39,156

P. putida (F)

43%

P. fluorescens (F)

17%

P. aeruginosa (F)

11%

7

7396 / 51,203

P. putida (F)

35%

P. aeruginosa (F)

9%

P. fluorescens (F)

7%

8

8931 / 50,297

P. putida (F)

39%

P. fluorescens (F)

23%

P. monteilii (G)

6%

9

6569 / 40,365

P. monteilii (G)

17%

P. putida (F)

15%

P. fluorescens (F)

9%

10

9391 / 53,610

P. putida (F)

38%

P. fluorescens (F)

22%

P. monteilii (G)

14%

11

9474 / 55,238

P. putida (F)

51%

P. koreensis (F)

16%

P. fluorescens (F)

8%

12

6920 / 43,695

P. putida (F)

49%

P. aeruginosa (F)

11%

P. fluorescens (F)

6%

  1. aEach sample is arranged in order of abundance from largest to smallest, and top 3 species are selected in every sample. The percentage after each specie is the abundance of the sample
  2. bGap or Frequency results can identify the sequence. The percentage is ratio of the ITS sequence of the species and the Pseudomonas ITS sequence