Table 3 Genomic position and primer sequences for the qRT-PCR assay of the 14 hypothetical and the aspC genes of the symbiosis island of Bradyrhizobium japonicum SEMIA 5079 (= CPAC 15) selected for this study
Locus | Protein ID | Physical Location | Primer Sequence ( 5’– 3’) | Amplicon bp size | |
|---|---|---|---|---|---|
bjs_07536 | AHY48760 | 415,677.415,677 (+) | F GTGTCGACCAACATTCATGC R TGGGATACAGCCACGAAAC | 142 bp | |
bjs_07605 | AHY48686 | 318,489.320,720 (+) | F GGCAAGTTGGGTTCAGTTTG R CTTTGCGAACCGGTTATAGG | 97 bp | |
bjs_07621 | AHY48670 | 287,116.288,339 (+) | F GCGGCGCTATCAAATTCTAC R ACGAGCAAGTGGACAAATCC | 100 bp | |
bjs_07647 | AHY48641 | 259,705.260,430 (-) | F TTTCGTCATGCGTTGTGG R ATTTCCGTCCTCATGCGTAG | 114 bp | |
bjs_08160 | AHY56892 | 9,349,925.9,350,872 (+) | F CGATCCGCGATATCTCATTC R ATCGCGACCTTGTTCATCTC | 111 bp | |
bjs_08216 | AHY48554 | 152,594.154,168 (+) | F GATCCACGGGATGTTTGAAG R CGCAGATCAATTCACGTAGC | 137 bp | |
bjs_08240 | AHY48531 | 125,139.126,544 (-) | F CCTTCGAGGACTTGATTTCG R GACGTGTCCGTTGAATTCTG | 107 bp | |
bjs_08251 | AHY48520 | 98,439.100,538 (+) | F CAACGATGATACGGTTGCAG R GCATGACCTTGATTGCCTTC | 131 bp | |
bjs_08254 | AHY48517 | 96,080.96,979 (+) | F GGTCGGCTATTTCAAGATCG R GATAGCCCGAAATGTTGACG | 74 bp | |
bjs_08258 (aspC) | AHY48514 | 91,505.92,803 (+) | F ATCCGAGCTGCCATTACATC R GCAATGTTGTTCGGCTGTAG | 91 bp | |
bjs_08261 | AHY48511 | 85,326.86,360 (+) | F CGTGATCAATCTCGTCTTCG R TGAAGGAGACTTTCCGGATG | 83 bp | |
bjs_08267 | AHY48505 | 76,029.77,225 (+) | F TCCTGATCTGGATGTCAACG R AGAAGCATGGGATGAGCAAC | 105 bp | |
bjs_08317 | AHY48455 | 18,261.20,504 (+) | F AAATAGTCCGGCTGACAAGG R CATAGGTCTTCGTGCAATCG | 102 bp | |
bjs_08523 | AHY56944 | 9,425,196.9,426,470 (+) | F TGAGAAGCAGGAAGAGTTCG R CTCCGAAGGCGAGAAATATG | 134 bp | |
bjs_08774 | AHY56918 | 9,392,008.9,393,012 (+) | F ACAAAGGGTGGCTCGAAAC R TCGTAAGCGCGTAGTAAACG | 138 bp | |