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Table 2 Comparison of recovery results of twelve strains of G. pectorale (Additional file 1: Table S1) after possible optimal cryogenic treatment (1.0 mL 6% DMF in 2 mL cryotube; Table 2) in liquid nitrogen

From: Cryopreservation of vegetative cells and zygotes of the multicellular volvocine green alga Gonium pectorale

Strain designation

Survivability based on MPN method [%]

Number of viable cultures by 1st [2nd] inoculation with 3 [3] 10 mL cultures

NIES-4499

1.7 ± 1.7

3 [3]

NIES-4500

0.19 ± 0.05

3 [3]

NIES-4501

0.1 ± 0.0

3 [3]

NIES-4502

2.7 ± 3.3 (Table 1)

3 [3]

NIES-2261 a

0.0018 ± 0.0008

3 [3]

NIES-2262

0.10 ± 0.046

3 [3]

NIES-4121

3.3 ± 1.3

3 [3]

NIES-468

0.28 ± 0.19

3 [3]

NIES-469 a

0.0013 ± 0.0023

2 [0]

NIES-569

0.28 ± 0.19

3 [3]

NIES-570 a

0.012 ± 0.008

3 [3]

NIES-645

4.2 ± 8.5

3 [3]

NIES-646

38 ± 28

3 [3]

  1. a Not used for cryopreserved strain in MCC-NIES because of < 0.1% MPN survivability