Table 1 Comparison of results of four types of cryopreservation conditions for vegetative cells of Gonium pectorale strain NIES-4502 based on most probable number (MPN) methods
Experiment I | Experiment II | ||
|---|---|---|---|
Conditions for cryopreservation | Total viabilitya (range) [%] | MPN cell numbers in three tubes (/mL) (control)b | MPN cell numbers in three tubes (/mL) (control)b |
0.2 mL 3% DMF in 0.2 mL PCR tube | 0.31 ± 0.67 (0.0016–1.8) | 7,2, 7.2, 7.2 (46,000) | 7.2, 18, 8400 (460,000) |
0.2 mL 6% DMF in 0.2 mL PCR tube | 0.38 ± 0.36 (0.031–1.0) | 460, 460, 840 (84,000) | 150, 220, 300 (460,000) |
1.0 mL 3% DMF in 2 mL cryotube | 2.1 ± 2.2 (0.04–10) | 300, 840, 3000 (46,000) | 180, 1800, 15,000 (460,000) |
1.0 mL 6% DMF in 2 mL cryotube | 2.7 ± 3.3 (0.65–10) | 460, 1500, 8400 (84,000) | 3000, 7600, 8400 (460,000) |