Fig. 3

Effect of recombinant proteins of M. agalactiae on the viability of eukaryotic cells. The sheep MSC and HeLa cells were seeded in 96-well plates and after overnight incubation treated with (A and D) 1, (B and E) 2 and (C and F) 4 μg.mL^− 1 of the P40, MAG_1560 or MAG_6130 proteins. After 48 h of treatment, alamarBlue™ reagent was added and its reduction was monitored spectrophotometrically at 570 nm and 600 nm. Statistical analysis was performed using Student’s t test. (*) p < 0.05, (**) p < 0.01 represent statistically significant differences