BMC Microbiology

Table 4 PCR Multiplex description

From: Using unique ORFan genes as strain-specific identifiers for Escherichia coli

Name of multiplex^a	Strains detected^b	Conditions
Name of multiplex^a	Strains detected^b	Step	Temp (°C)	Time (s)
Chicken-Human	ChH1	Initial denaturation	94	30
	ChH2	30 cycles	94	30
	ChH3		48	40
			68	60
		Final extension	68	300
Cattle	C1	Initial denaturation	94	30
	C2	30 cycles	94	30
	C3		52	60
	C4		68	70
	C5	Final extension	68	300
	C6	Final extension	68	300
Pig	P1	Initial denaturation	94	30
	P2	30 cycles	94	30
	P3		51	35
	P4		68	40
	P5	Final extension	68	300
Mix	M1	Initial denaturation	94	30
	M2	30 cycles	94	30
	M3		49	40
			68	45
		Final extension	68	300

^aFrom now on, referred to as Chicken-Human multiplex, Cattle multiplex, Pig multiplex and Mix multiplex in the text
^bStrain information displayed in detail in Table 1

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ISSN: 1471-2180

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