Using unique ORFan genes as strain-specific identifiers for Escherichia coli

BMC Microbiology

Table 2 List of strain-specific PCR primers

Strain^a	Gene locus tag	Primers		Ann. T° (°C)	Product (bp)
Strain^a	Gene locus tag	Forward (5′ → 3′)	Reverse (5′ → 3′)	Ann. T° (°C)	Product (bp)
ChH1	ECMDIDHM_04458	GGAAACGGATTACTTCTACG	CTGATAGAGATTCAGTCCCC	48	793
ChH2	ANLLMAEJ_01457	CCTACGCCACTAAACTACTG	CTATCATCACTGGAAATCCTG	47	560
ChH3	PDAKODAI_04433	GAATTACCGTCGTAGAGCAG	GGTATGGACTCAATGACAC	47	970
C1	KGJMKMMK_04916	CTGCTGTTATTAATCGGCTTGG	CTCTCAAGCGTGCTTTCTATC	51	886
C2	KGPFHGEA_00668	GTGGTGTAGAATTTATCGCATCC	AGCCATTCGATGAAACCAAG	51	665
C3	AMKFMELI_04046	GGGTCGATAACTTAGCAAGC	CTTGTTTGCAGAATGCTGCG	51	465
C4	LCFLNNEA_01211	GAATGGCAGGGCATACAAG	CAGACCTATGAACCTCTCCC	52	522
C5	GKDLAHGH_04328	CAGGAATGACAAACCTCTCG	CCGAACCATCGATTTGTCTG	51	1097
C6	LDHELLGP_02401	CTGCGGAAGAGTGTAAGTTC	CAGCGTCATCACTAAGCATT	51	162
P1	NIHDEHIB_01629	GTTAGCGGAACTCCAGCGGA	CCTCAGGTGCTTACTACGTTC	53	178
P2	EKBHOPAC_04494	CCAGATAGAGTCGTTTCTGC	GCAGCATTAACAGTAGGTCC	50	434
P3	DGMDDDKP_04627	GGCGATACGATTTTAACACCA	GCAACGGTCTAACATTCGCTG	50	347
P4	LLPKFPJB_0002	GATATAGCAAAAGCCGTTTCCTG	GCCTAGCAATAAATAACCGGTC	51	564
P5	HEMKEPCD_04830	GCTCGACATATTCCGAACAG	CACAGTTCTGGTGCAATAGAG	51	267
M1	PJHDGJGH_02587	GGCCATTGATAGCAGCATTG	CCGAATAATAACCATCGCC	48	371
M2	HDADFJHI_04570	CAGTTATGCTGGGCTAATTG	TGCGTAATTTGCATGATATGG	48	485
M3	EBAAKEFM_00440	CAGCAACGGATTGATACCTC	GCGAAGTTCTTCAATCTCC	48	697

ISSN: 1471-2180