Fig. 1
From: Using unique ORFan genes as strain-specific identifiers for Escherichia coli
Results of the multiplexes designed and their specificity for the target strains. DNA was isolated from each bacterial strain and tested with each of its corresponding multiplexes. Gel A – Chicken-Human multiplex: strains ChH1 (1), ChH2 (2), ChH3 (3); Gel B – Cattle multiplex: C1 (1), C2 (2), C3 (3), C4 (4), C5 (5), C6 (6); Gel C – Pig multiplex: strains P1 (1), P2 (2), P3 (3), P4 (4), P5 (5); Gel D – Mix multiplex strains M1 (1), M2 (2), M3 (3). Lane N: Negative control; lane M: 100-bp marker (New England Biolabs Inc., Ipswich, MA, USA)