Fig. 7

Gene Set Enrichment Analysis highlights decrease of genes related to flagellar assembly. A Differential gene abundance between sibutramine-treated rats at the start (day − 3) and end (day 42) of the treatment. Heatmap representing log2-transformed and normalized read counts for each of the 1047 differentially abundant genes. As with th species in Fig. 6, only genes with adjusted P value <= 0. 05 were considered statistically significant. Top bar shows which columns correspond to Day − 3 (green) or Day 42 (blue) samples. B Enrichment plot for the pathway “Flagellar Assembly” (ko:02040). Briefly, genes have been ranked by log2 fold change multiplied by the -log10(p.adjusted), then gsea was performed with the fgsea R/Bioconductor package [50]. “Flagellar Assembly” was the only statistically significant result (normalized Enrichment Score: − 1.7, p.adjusted = 0.03). C Schematic representation of the bacterial flagellum and its protein components. Genes detected in our analysis are indicated [51]. D Boxplots of leading edge genes belonging to the bacterial flagellum pathway, all of which are differentially abundant in sibutramine samples at Day 42 compared to Day − 3. Boxplots were obtained by plotting the log2-normalized expression values for each gene