Fig. 6
From: LysX2 is a Mycobacterium tuberculosis membrane protein with an extracytoplasmic MprF-like domain
1D-Thin layer Chromatography (TLC) and MALDI-TOF mass spectrometry analysis of total lipid extracts from M. smegmatis MS321 (mc2155::pROL_Hyg ) and MS322 (mc2155::MtlysX2) grown at pH 6.8 and pH 5.5. A Revelations of the same plate were performed using different dyes: ninhydrin for free amino groups, Dittmer reagent for phosphate esters and anthrone for glycolipids. Solvent: CHCl3/CH3OH/H2O (60:35:8 v/v/v). Standards: LPG (Lysyl-PG), PI (phosphatidyl inositol), PG (phosphatidyl glycerol), CL (cardiolipin), PE (phosphatidyl ethanolamine), PIM (phosphatidylinositol mannosides). B Glycopeptidolipids (GPLs) were analyzed by employing CHCl3/CH3OH (90:10 v/v) as running solvent. The GPLs were detected with anthrone reagent and appear as blue spots (CF : cord factor). (C) MALDI-TOF mass spectra of the crude GPL fractions (GPL I : apolar diglycosylated GPLs ; GPL IIIa : succinylated diglycosylated GPLs ; GPL IIIb : succinylated triglycosylated GPLs)