Fig. 2

Membrane topology of MtLysX2. A Schematic representation of MtLysX2 (Rv1619) membrane topology as predicted by both HMMTOP and TMHMM. The MprF-like domain with sequence similarity to the Lys-PG synthase domain of SaMprF is highlighted in dark gray and pointed by a thick gray arrow. Thin arrows indicate the points chosen for fusions with either lacZ or phoA. B Schematic representation of the three different fragments of MtLysX2 (black) fused to either phoA or lacZ (white) and their relative activities, representative of two independent experiments. IN: inside the cytoplasm. OUT: outside the cytoplasm. The phoA/lacZ moiety is not drawn on scale as the two genes have a different length. Numbers indicate amino acid positions. β-galactosidase activities (β-gal) are normalized relative to that of fusion A. Alkaline phosphatase activities (AP) are normalized relative to that of fusion B