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Table 4 Cycles and temperature in RT-PCR assay for quantitative assessment of immune genes

From: Effects of probiotics on Zebrafish model infected with Aeromonas hydrophila: spatial distribution, antimicrobial, and histopathological investigation

Step

Temperature

Time

Cycles

Initiation

95

15 min

One

Denaturation

95

15 s

40

Ligation

60

30 s

40

Elongation

60

30 s

40

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BMC Microbiology

ISSN: 1471-2180

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