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Table 4 Cycles and temperature in RT-PCR assay for quantitative assessment of immune genes

From: Effects of probiotics on Zebrafish model infected with Aeromonas hydrophila: spatial distribution, antimicrobial, and histopathological investigation

Step Temperature Time Cycles
Initiation 95 15 min One
Denaturation 95 15 s 40
Ligation 60 30 s 40
Elongation 60 30 s 40