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Fig. 5 | BMC Microbiology

Fig. 5

From: Reliable detection of Burkholderia pseudomallei using multiple cross displacement amplification label-based biosensor

Fig. 5

Optimal duration of time required for B. pseudomallei-MCDA-NB assay. Four different reaction times (Biosensor 1, 10 min; Biosensor 2, 20 min; Biosensor 3, 30 min; and Biosensor 4, 40 min) were examined and compared at 66°C. B. pseudomallei-MCDA reactions were performed using the LoD level of templates (10 fg per reaction), and the templates at the LoD level were able to been detected when the isothermal amplification only lasted for 30 min (Biosensor 3)

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BMC Microbiology

ISSN: 1471-2180

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