Fig. 1

Southern blotting analyses of the wild-type Streptomyces sp. 139 and Strain D2. A Representative of the construction of the ste2 disruption strain D2. B (Line 1) Streptomyces sp. 139 chromosome DNA digested with BamHI and HindIII, (Line 2) Strain D2 chromosome DNA digested with BamHI, a DIG-labeled F1 as the hybridization probe. kan: kanamycin resistance gene fragment