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Table 2 Comparison of semi quantitative culture and RT-PCR results

From: Comparison of pneumococcal colonization density among healthy children and children with respiratory symptoms using real time PCR (RT-PCR)

Culture result

RT-PCR positive (Cq =  < 35) (n = 124)

RT-PCR negative (Cq > 35) (n-160)

No S. pneumoniae isolated (n = 167)

21 (12.6%)

146 (87.4%)

Heavy growth of S. pneumoniae isolated (n = 31)

31 (100%)

0

Moderate growth of S. pneumoniae isolated (n = 27)

25 (92.6%)

2 (7.4%)

Scanty growth of S. pneumoniae isolated (n = 59)

47 (79.7%)

12 (20.3%)

  1. *Percentages given with the culture result as the denominator