Table 1 Bacterial strains and plasmids used in this study
From: The global regulators ArcA and CytR collaboratively modulate Vibrio cholerae motility
Strain or plasmid | Characteristicsa | Reference or source |
|---|---|---|
Vibrio cholerae | ||
 El2382 | Virulent strain, O1 El Tor type, PmBr | Shanghai Municipal Center for Disease Control & Prevention |
 ΔarcA | El2382, deletion of arcA, PmBr | This study |
 ΔarcA::ParcA | ΔarcA containing pBAD33 carrying arcA ORF with its own promoter, PmBr | This study |
 ΔcytR | El2382, deletion of cytR, PmBr | This study |
 ΔcytR::PcytR | ΔcytR containing pBAD33 carrying cytR ORF with its own promoter, PmBr | This study |
Escherichia coli | ||
 S17-1 (λpir) | Tpr Smr recA thi pro rK- mK- RP4:2-Tc:MuKm Tn7 λ pir (thi pro hsdR hsdM + recA RP4-2-Tc:: Mu-Km-Tn7) | [1] |
 BL21(DE3) | Host strain for protein expression | This study |
 BL21/pET28a::arcA | BL21(DE3) with pET28a carrying the arcA ORF, Kmr | This study |
 BL21/pET28a::cytR | BL21(DE3) with pET28a carrying the cytR ORF, Kmr | This study |
 BL21/pET28a::crp | BL21(DE3) with pET28a carrying the crp ORF, Kmr | This study |
Plasmids | ||
 pRE112 | pGP704 suicide plasmid, pir dependent, oriT, oriV, sacB, Cmr | [2] |
 pBAD33 | arabinose inducible promoter, Cmr | [3] |
 pET28a | Expression vector, Kmr | This study |
 pET28a::arcA | pET28a carrying the arcA ORF, Kmr | This study |
 pET28a::cytR | pET28a carrying the cytR ORF, Kmr | This study |
 pET28a::crp | pET28a carrying the crp ORF, Kmr | This study |
 pMS402 | For construct promoter-luxCDABE reporter fusion; Kmr | This study |
 cytR-lux | pMS402 carrying the cytR promoter region, Kmr | This study |
 flrA-lux | pMS402 carrying the flrA promoter region, Kmr | This study |
 fliK-lux | pMS402 carrying the fliK promoter region, Kmr | This study |