Fig. 3

Protist communities in dairy cattle manure before and after aging and house fly larval grazing. a) Protist OTUs in Fresh, Aged, and house fly larval grazed (Grazed) manure types. Overlapping sections present the number of OTUs shared between and/or across manure types, and non-overlapping are unique to the manure type. Each manure type consisted of 4 replicates except fresh which included 8 replicates; b) Mean relative abundance of phyla in three manure types; c) Effect of manure types on the relative abundance of protist taxa: Buxtonella, Enteromonas, Trepomonas, Trimitus, Tetratrichomonas, Hexamitinae (unclassified Hexamitinae-Enteromonadida), Oxytricha, Cercomonas, unclassified Nolandellidae, unclassified Euglyphida, unclassified Thraustochytriaceae, Filosa (unclassified Filosa-Sarcomonadea), Colpodella, Vannella, Parabodo and unclassified Chrysophyceae. In the box plots, median and interquartile range (25th–75th percentiles; boxes) with upper and lower range of values are shown. The different letters on top of each box indicate the significant differences between manure types (p ≤ 0.05)