Table 1 Bacteria and plasmids used in this study

 1830

pro⁻met⁻Km^rNm^r, containing transposon

Gantotti et al. [40]

Tn5 on the “sucidal” plasmid pJB4JI

 DH5α

supE44hsdR17recA1endA1gyrA1thi-1relA1

Hanahan; Reusch et al. [60, 61]

 89-H-4

putative biocontrol agent

Laboratory stock

  H-rif-8-6

89-H-4, Rif^r

this work

  Ea1068

wild type

Laboratory stock

  SP33

wild type

Laboratory stock

  TH22–6

H-rif-8-6, sctT::Tn5, Rif^r, Kan^r

this work

  FlhA-KO

H-rif-8-6, flhA::Kan, Rif^r, Kan^r

Chan et al. [9]

  SctV-KO

H-rif-8-6, sctV::Kan, Rif^r, Kan^r

this work

  SctU-KO

H-rif-8-6, sctU::Kan, Rif^r, Kan^r

this work

  SctD-KO

H-rif-8-6, sctD::Kan, Rif^r, Kan^r

this work

  SctQ-KO

H-rif-8-6, sctQ::Kan, Rif^r, Kan^r

this work

  FlgH-KO

H-rif-8-6, flgH::Kan, Rif^r, Kan^r

this work

  FlgF-KO

H-rif-8-6, flgF::Kan, Rif^r, Kan^r

this work

  FlhB-KO

H-rif-8-6, flhB::Kan, Rif^r, Kan^r

this work

  FliR-KO

H-rif-8-6, fliR::Kan, Rif^r, Kan^r

this work

  FliG-KO

H-rif-8-6, fliG::Kan, Rif^r, Kan^r

this work

  FliE-KO

H-rif-8-6, fliE::Kan, Rif^r, Kan^r

this work

  FlgG-KO

H-rif-8-6, flgG::Kan, Rif^r, Kan^r

this work

  pACYC177

Amp^r,Kan^r, low copy number

Chang et al. [62]

  pBR322

Amp^r,Kan^r

Bolivar et al. [38]

  pB47

Amp^r,Kan^r, 3.0 kb DNA fragment contain sctT

this work

  pBSCTT

Amp^r, sctT

this work

  pBFA

Amp^r, flhA

this work