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Fig. 1 | BMC Microbiology

Fig. 1

From: Injectisome T3SS subunits as potential chaperones in the extracellular export of Pectobacterium carotovorum subsp. carotovorum bacteriocins Carocin S1 and Carocin S3 secreted via flagellar T3SS

Fig. 1

Bacteriocin activity of P. carotovorum subsp. carotovorum. The bacteriocin production of the test strains was examined by the double-layer method [38, 39]. Numbered strains: 1, Escherichia coli pJB4JI (containing Tn5)/1830; 2, H-rif-8-6 (wild type, parent); 3, TH22–6 (the transposon Tn5 insertional mutant); 4, TH22–10 (the transposon Tn5 insertional mutant for carocin S1) [20]. The indicator organism used in the agar overlay was Pcc strain Ea1068. The red colony, Serratia marcescens, was used as a marker, to indicate the direction and location of numbered colonies. The result showed that TH22–6 strain loses the low-molecular-weight bacteriocin activity to Ea1068

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BMC Microbiology

ISSN: 1471-2180

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