Fig. 1

The result of PCR verification for wild-type strain (wt) and ccpA-knockout strain (ko) of S. bovis S1. A Diagram of PCR verification for genomic structure of wild-type strain (wt) and ccpA-knockout strain (ko). ccpA 1 was designed based on the internal sequence of the ccpA gene, with a size of about 426 bp; ccpA 2 was designed across the upstream and downstream sequences of the ccpA gene, with a size of about 1827 bp for wild-type strain and 1985 bp for ccpA-knockout strain. B The result of the PCR verification. wt1: a 426 bp PCR product of ccpA 1 amplified in wild-type strain; ko1: no PCR product of ccpA 1 amplified in ccpA-knockout strain; wt2: a 1827 bp PCR product of ccpA 2 amplified in wild-type strain; ko2: a 1985 bp PCR product of ccpA 2 amplified in ccpA-knockout strain