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Fig. 2 | BMC Microbiology

Fig. 2

From: IgG antibody titers against SARS-CoV-2 nucleocapsid protein correlate with the severity of COVID-19 patients

Fig. 2

A western blot for the purified rSARS-CoV-2-N protein. The prestained protein marker and purified recombinant proteins were separated by SDS-PAGE and transferred to a PVDF membrane. Each membrane was incubated with diluted patient’s serum or mouse immune serum, followed by horseradish peroxidase conjugated-goat anti-human IgG or anti-mouse IgG (1:1000 dilution), and detected by DAB staining. (A) Reactivity of recombinant proteins to COVID-19 patient serum (1:400 dilution). Lane 1: protein marker; Lane 2: purified rSARS-CoV-2-N protein. (B) Reactivity of recombinant proteins to rSARS-CoV-2-N-immunized mouse serum (1:800 dilution). Lane 1: protein marker; Lane 2: purified rSARS-CoV-2-N protein

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BMC Microbiology

ISSN: 1471-2180

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